BIOLOGY

I. Introduction: (1 page).

A. Introduction to background information about erythrocytes in general and membrane structure, composition, and the fluid mosaic model
B. Experimental goals of the lab.
C. What use, clinical or research, could the information from these types of experiments be put to?
D. Brief descriiption of the general procedural approach used for each goal.

II. Materials and methods: (1 page)

Give a BRIEF synopsis in paragraph form of just the essential concepts of each procedure. Explain it as if you were trying to explain what you did to a scientist who already understands the techniques involved. Do not give a narrative of how the procedure was performed or a numbered list of the steps: DO NOT just retype the lab handout procedure. Include important specific information: blood cells, reagents, centrifuge g-forces and times, and what calculations were performed. Also, include any errors or changes to the procedures.

As briefly as possible, recount the methods used to:

i. Isolate Membrane
ii. determine surface carbohydrate (Hemagglutination assay)
iii. determine Lipid types (TLC)
iv. determine membrane protein asymmetry and types (labeling and gel)
v. lipid/protein ratio (gravimetric analysis and Lowry)

WRITE THE ANSWER TO THE INTRODUCTION AND MATERIALS/METHODS IN THE LAB REPORT ATTACHED. ALSO, I ADDED THE LAB MANAUL WITH FULL STEPS ON WHAT THIS REPORT WAS ABOUT IF YOU HAVE ANY QUESTIONS.

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